The Two-Spirit identity is a culturally significant term used by some Indigenous North American communities to describe a person who embodies both masculine and feminine spirits. This identity is not solely about gender or sexual orientation but is deeply rooted in Indigenous traditions and spiritual beliefs. Two-Spirit people often hold unique roles within their communities, serving as healers, mediators, or keepers of tradition. The term is a modern, pan-Indigenous concept that encompasses a variety of gender identities and sexual orientations, acknowledging the diversity and fluidity of these experiences within Indigenous cultures. It is important to recognize that the Two-Spirit identity is specific to Indigenous peoples and should be understood within the context of their cultural heritage and history.

The Two-Spirit Pride Flag features a combination of colors and symbols that reflect the rich cultural heritage and diverse identities within the Two-Spirit community. The flag typically includes a rainbow to represent the spectrum of gender identities and sexual orientations, acknowledging the connection to the broader LGBTQ+ community. Additionally, the flag often incorporates traditional Indigenous symbols, such as feathers or circles, which signify the spiritual and cultural aspects of the Two-Spirit identity. Each element of the flag is thoughtfully chosen to honor the unique blend of gender, sexuality, and cultural identity that Two-Spirit individuals embody, serving as a visual representation of their pride and resilience.